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. 2018 Jan 26;50(1):e433. doi: 10.1038/emm.2017.246

Figure 3.

Figure 3

Hydrogen-water upregulates the level of MyD88-targeting miR-1968-5p in the mouse small intestines. (a) The expression level of MyD88 was examined in the aforementioned small intestine tissues by qRT-PCR. Statistically significant differences are indicated: **P<0.01; ***P<0.005; Student’s t-test. (b) The expression of MyD88 was examined by western blotting in TAI-exposed mice with or without hydrogen-water treatment. (c) Alterations in miRNA expression in small intestine tissues from mice without or with TAI were assessed using microarray analysis. (d) The expression level of miR-1968-5p was examined by qRT-PCR in small intestine tissues from mice without TAI (Control, n=20), mice with TAI (TAI, n=20) and mice with hydrogen-water treatment after TAI (TAI+HW, n=20) individually. Statistically significant differences are indicated: *P<0.05; **P<0.01; Student’s t-test. (e) The correlation between MyD88 mRNA expression and miR-1968-5p level was examined by qRT-PCR in 20 cases of small intestine tissues from mice with hydrogen-water treatment after TAI. **P<0.01; Pearson correlation coefficient, r=−0.7296. (f) The expression level of TLR4 was examined in the aforementioned small intestine tissues by qRT-PCR. Statistically significant differences are indicated: *P<0.05; ***P<0.005; Student’s t-test. (g) The expression level of TLR5 was examined in the aforementioned small intestine tissues by qRT-PCR. Statistically significant differences are indicated: *P<0.05; Student’s t-test.