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. 2018 Jan 26;50(1):e433. doi: 10.1038/emm.2017.246

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Hydrogen-water treatment has no effect on the abundance of enteric bacteria. (a) The observed species number of intestinal bacteria in con and TAI-treated mice (with or without hydrogen-water oral gavage) was examined by 16S rRNA high-throughput sequencing after 5 days of TAI exposure. (b and c) The Shannon (b) and Simpson (c) diversity indices of intestinal bacteria in con and TAI-treated mice (with or without hydrogen-water oral gavage) were assessed by 16S rRNA high-throughput sequencing after 5 days of TAI exposure. For panels (a–c), the top and bottom boundaries of each box indicate the 75th and 25th quartile values, respectively, and lines within each box represent the 50th quartile (median) value. Ends of whiskers mark the lowest and highest diversity values in each instance. (d) The relative abundance of enteric bacteria at the phylum level in con and TAI-treated mice (with or without oral gavage of hydrogen-water) was assessed using 16S high-throughput sequencing after irradiation at day 5. Statistically significant differences are indicated: Student’s t-test, n=4 per group.