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. 2018 Jan 26;50(1):e434. doi: 10.1038/emm.2017.247

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Knockdown of rpS3 enhances the resistance of GBM cells to irradiation. (a) SiRNA knockdown efficiency of rpS3 in U87MG and A172 cells was analyzed by western blotting. (b) Short-term effects of rpS3 knockdown on cell growth in both U87MG and A172 cells following irradiation (IR) were assessed via MTT assay. The data are represented as the mean±s.e.m. (n=3); *P<0.05 compared with non-irradiated cells, **P<0.05 compared with cells treated with irradiation-alone or cells treated with irradiation and scrambled siRNA. (c) Long-term effects of rpS3 knockdown on cell growth in both U87MG and A172 cells following radiation exposure were assessed via colony forming assay. The data are represented as the mean±s.e.m. (n=3); *P<0.05 compared with non-irradiated cells, **P<0.05 compared with cells treated with irradiation-alone or cells treated with irradiation and scrambled siRNA. (d) Radiation-induced translocation of rpS3 from the cytoplasm to the nucleus was assessed by western blotting after cytoplasmic or nuclear fractionation (CE, cytoplasmic extract; NE, nuclear extract). Tubulin and Lamin A/C were used as markers for the CE and NE, respectively. (e) Radiation-induced translocation of rpS3 from the cytoplasm to the nucleus was assessed by IF staining. Scale bars, 25 μm.