Table 1. Collision Energy (NCE), Sequence Coverage, and Percentage of Explained Fragments for the HCD and ECD Fragmentation of Ubiquitin and Myoglobin.
analyte | NCE | % sequence coverage | % fragments explained |
---|---|---|---|
ubiquitin | 15 | 53 | 25 |
18 | 67 | 23 | |
20 | 69 | 20 | |
22 | 75 | 14 | |
25 | 81 | 11 | |
ECD | 80 | 69 | |
myoglobin | 15 | 45 | 23 |
18 | 48 | 13 | |
20 | 43 | 10 | |
22 | 28 | 5 | |
25 | 18 | 3 | |
ECD | 60 | 74 |