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. 2017 Dec 5;139(50):18365–18375. doi: 10.1021/jacs.7b10702

Figure 3.

Figure 3

Site-selective modification of C2Am-Dha95 with a range of amines. (a) The reaction of C2Am-Cys95 with a wide variety of N-nucleophiles. The conversions listed here are the maximum conversions that could be obtained by reacting the different N-nucleophiles with C2Am-Dha95. Further experimental details on each reaction may be found in Supporting Information. (b) The reaction of compound 26 with C2Am-Dha95 went to completion as detected by ESI–MS shown here. (c) Treatment of C2Am-Dha95 with compound 27 afforded a fluorescent protein conjugate, as detected by SDS-PAGE gel shown here. Lanes 1 and 2, Coomassie staining. Lanes 3 and 4, fluorescence.