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. 2018 Feb 5;9:30. doi: 10.1186/s13287-018-0788-2

Table 1.

Primers used for reverse transcription-polymerase chain reaction to establish the undifferentiated state of mouse embryonic stem cell line E14TG2A

Gene product Sense primer sequence (orientation: 5′–3′) Antisense primer sequence (orientation: 3′–5′) Size (base pairs)
Oct3/4 AGCCTGAGGGCGAAGCAGGA CCCCAGGGTGAGCCCCACAT 236
Sox2 AGCTACAGCATGATGCAGGA GGTCATGGAGTTGTACTGCA 126
Nanog CAGCCCTGATTCTTCCACCAGTCCC TGGAAGGTTCCCAGTCGGGTTCACC 391
β-actin CATCACTATTGGCAACGAGCG ATGGATGCCACAGGATTCCA 85