Fig. 4.

a Percentage of reads mapping to Brassica napus miRNAs. The proportion of reads mapping to B. napus (oilseed rape) miRNAs (with 2’ OMe modification) in miRBase were determined for TruSeq protocols TS1-, 5 and 7, and the NEXTflex protocols (Nf1, 2 and 6). We calculated the percentage of the total numbers of raw reads (red bars) or the total numbers of reads after trimming (blue bars) that mapped to miRNAs. Shown are the mean values of at least two independent experiments and the error bars represent standard deviations. b Numbers of B. napus miRNAs identified. We determined the numbers of known miRNAs identified with the different protocols. For each protocol, 0.5 million reads were mapped to B. napus miRNAs in miRbase (92 in total). Shown are the mean values of at least two independent experiments with standard deviations represented by error bars