Table 4.
Single Nucleotide Polymorphism (SNP) and association analysis with susceptibility to severe malaria (SM)
| NCBI dbSNP number | Phénotype | MAF | Nominal p values for statistical test (additive models) | |||||
|---|---|---|---|---|---|---|---|---|
| SM | UM | CTR | All | SM vs. UM | UM vs. CTR | SM vs. CTR | ||
| A. Hb S and Hb C polymorphisms and association with SM | ||||||||
| rs334 A > T OR (95% CI) |
HbS | 0.026 | 0.069 | 0.035 | 0.050 |
0.033
2.67 (2.01–3.5) |
0.257 | 0.446 |
| rs33930165 G > A OR (95% CI) |
HbC | 0.000 | 0.009 | 0.029 | 0.011 | 0.258 | 0.1661 |
0.0084
1.67 (0.99–3.42) |
| rs713040 C > T OR (95% CI) |
0.107 | 0.068 | 0.119 | 0.099 | 0.097 |
0.029
2.33 (1.55–7.33) |
0.660 | |
| RNASE3 SNPs | Nominal p values for statistical test | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Position (NCBI Ids) | Associated allele | UM vs. CTR | SM vs. CTR | SM vs. UM | SM vs. UM + CTR | ||||
| ADD | D/R | ADD | D/R | ADD | D/R | ADD | D/R | ||
| B. Association analysis with susceptibility to severe malaria under Hb polymorphisms corrections | |||||||||
| Promoter −550G/A (rs2284954) | A | 0.30 | 0.81 | 0.71 | 0.84 | ||||
| Promoter −399T/C (rs147413155) | C | 0.36 | 0.28 | 0.55 | 0.23 | ||||
| Promoter −38C/A (rs2233859) | A | 0.38 | 0.29 | 0. 98 | 0.47 | ||||
| Coding +371_G/C (rs2073342) OR (95% CI) |
G | 0.08 1.8 (0.35–0.91) |
0.01 (D)
3.9 (1.29–11.7) |
0.05
1.78 (0.99–3.1) |
0.08 (D)
2.18 (0.88–5.36) |
0.86 | 0.17 | ||
| 3′utr + 499_G/C (rs2233860) OR (95% CI) |
C | 0.52 |
0.05
1.46 (1.21–2.09) |
0.01 (D)
1.30 (1.11–1.79) |
0.003
1.43 (1.20–1.92) |
0.01 (D)
1.28 (1.10–1.76) |
0.001
1.50 (1.30–1.84) |
0.004 (D)
1.29 (1.21–1.74) |
|
| 3′utr + 577_A/T (rs8019343) | T | 0.66 | 0.16 | 0.17 | 0.06 1.83 (0.96–3.05) |
0.07(D) 1.98 (0.92–4.32) |
|||
For each polymorphism, the p values for statistical tests were indicated. Statistical tests used were logistic regression analysis. (A) Association analysis for HB polymorphisms by comparison of SM vs Controls group B) RNASE3 polymorphisms association analysis adjusted for HbS polymorphisms (rs334 A > T) (B). The associated allele of polymorphism is given. The calculation modes for logistic regression test are indicated using different models (ADD additive, D Dominant, R Recessive modes). Borderline (0.05 ≤ p ≤ 0.1) and significant (0 ≤ p ≤ 0.05) p values are in bold. The p values for the dominant/recessive mode were showed only when lower than 0.1. (* Bonferroni corrections were not performed on p values). The OR (odds ratio) and CI (Confidence intervals) were showed when p values are significant or in borderline and were reprented in italic