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. 2018 Feb 5;18:52. doi: 10.1186/s12906-018-2119-7

Table 1.

PCR primer sequences for TGF-β1, Smad2, Smad3, SnoN and GAPDH

Gene Forward sequence Reverse sequence
TGF-β1 5’-ATACGCCTGAGTGGCTGTCT-3’ 5’-TGGGACTGATCCCATTGATT-3’
Smad2 5’-TGAGCTTGAGAAAGCCATCA-3’ 5’-TGTGTCCCACTGATCTACCG-3’
Smad3 5’-CATTACCATCCCCAGGTCAC-3’ 5’-CGTAACTCATGGTGGCTGTG-3’
SnoN 5’-CCATTCAATGCCCCATCCT-3’ 5’-AGTTCGTGGCCGCAATAAAG-3’
GAPDH 5’-CCTGCCAAGTATGATGACATCAAGA-3’ 5’-GTAGCCCAGGATGCCCTTTAGT-3’

GADPH, glyceraldehyde-3-phosphate dehydrogenase; Smad2, Sma- and Mad-related protein 2; Smad3, Sma- and Mad-related protein 3; SnoN, ski-related novel protein N; TGF-β1, transforming growth factor-β1