Table 2.

Primers used for real-time quantitative PCR

	Forward sequence (5′–3′)	Reverse sequence (5′–3′)
GAPDH	GAAGGTGAAGGTCGGAGTCA	AATGAAGGGGTCATTGATGG
SOX2	GAGAGTGTTTGCAAAAGGGG	TGGGGCTCAAACTTCTTCTC
SOX2 (2)	CCACCTACAGCATGTCCTACTCG	GGGAGGAAGAGGTAACCACAGG
OCT4	GCAGAAGAGGATCACCCTGG	AAAGCGGCAGATGGTCGTTT
OCT4 (2)	CTGCACAGATATGCAAAGCAG	TGATCTGCTGCAGTGTGGGT
NANOG	CTTGCCTTGCTTTGAAGCAT	TTCTTGACCGGGACCTTGTC
NANOG (2)	ACCTCAGCTACAAACAGGT	AAAGGCTGGGGTAGGTAGGT
hTERT	CGGAAGAGTGTCTGGAGCAA	GGATGAAGCGGAGTCTGGA
hTERT splicing variant	GCC TGA GCT GTA CTT TGT CAA	CGC AAA CAG CTT GTTCTC CAT GTC
ERK1/2 (MAPK1)	GCTAGATTCCAGCCAGGATACA	AGAACACCGATGTCTGAGCA
AKT1	ATGAGCGACGTGGCTATTGT	CCTCACGTTGGTCCACATCC
CSHL1	CTGTGGACAGCTCACCTAGC	AGCCTGGATAAGGGAACGGT
SMAD2	GTGGCAGGCGGGTCTAC	GCAAGCCACGCTAGGAAAAC
SMAD8	GTGGCCAACCTGTAGATGCC	CTCCCCAACTCGGTTGTTCA
β-Catenin	GGAGGAAGGTCTGAGGAGCAG	ATTGTCCACGCTGGATTTTCAA
SMAD1/5/9	CAGAGTGGCCAACCTGTAGA	TCCCCAACTCGGTTGTTCAG

GAPDH glyceraldehyde 3-phosphate dehydrogenase, SOX2 sex determining region Y-box 2, OCT4 octamer-binding transcription factor 4, hTERT human telomerase reverse transcriptase, ERK extracellular signal-regulated kinase 2, also known as mitogen-activated protein kinase 1 (MAPK1), AKT protein kinase B, CSHL chorionic somatomammotropin hormone like 1, SMAD, mothers against decapentaplegic homolog