Table 3. Steady State Kinetic Parameters for Unlabeled and Labeled FDHs at 25 °C.

	Light FDH	Half-Heavy FDH	Heavy FDH
kcatformate a (s-1)	1.78 ± 0.03	0.85 ± 0.04	0.92 ± 0.02
Kmformate a (mM)	2.2 ± 0.2	8.3 ± 1.3	4.5 ± 0.4
(kcat/Km)formate a (s-1 M-1)	(8.2 ± 0.6) × 102	(1.0 ± 0.1) × 102	(2.1 ± 0.2) × 102
kcatNAD+ b (s-1)	2.03 ± 0.05	0.79 ± 0.02	1.04 ± 0.03
KmNAD+ b (mM)	(2.1 ± 0.3) × 10-2	(5.9 ± 0.6) × 10-2	(6.1 ± 0.7) × 10-2
(kcat/Km)NAD+ b (s-1 M-1)	(9.7 ± 1.1) × 104	(1.3 ± 0.1) × 104	(1.7 ± 0.2) × 104

^ak_cat^formate, K_m^formate and (k_cat/K_m)^formate are turnover number, Michaelis constant and catalytic efficiency for formate;

^bk_cat^NAD+, K_m^NAD+ and (k_cat/K_m)^NAD+ are turnover number, Michaelis constant and catalytic efficiency for NAD⁺.