Figure 4.
Light chain subclass does not alter the interaction of IgG to FcRn. IgG molecules from panel 2, containing kappa or lambda light chains all paired with the same heavy chain subclass (IgG1), was compiled and tested for affinity to FcRn in the SPR assay a minimum of two times in duplicate on two different surfaces in two separate experiments. The mean steady-state KD values for each IgG molecule were plotted with error bars representing standard deviations. Kappa and lambda containing IgG molecules are colored differently. Significant steady-state KD differences between IgG molecules with the same light chain isotype were analyzed using an unpaired student t-test where significance is indicated as single asterisk (*) for p < 0.05.