Figure 5.
Variable domain FW family does not alter the interaction of IgG to FcRn. IgG molecules from panel 3, containing different variable domain FW families all paired with identical heavy and light chain subclasses, were compiled and tested for affinity to FcRn in the SPR assay a minimum of two times in duplicate on two different surfaces in two separate experiments. Mean steady-state KD values for each IgG molecule was plotted with error bars representing standard deviations. Different FW family containing IgG molecules are colored differently. Significant steady-state KD differences between IgG molecules with the same FW family were analyzed using an unpaired student t-test where significance is indicated as single asterisk (*) for p < 0.05. A. Heavy chain variable FW domain families VH1 and VH3. B. Light chain variable FW domain families VK1 and VK2, and VL1 and VL3.