Table 1.
Immobilized antigen-IgG complexes have reduced affinity for FcRn than uncomplexed IgG molecules. Steady-state KD affinity values were obtained from antigen-complexed IgG and uncomplexed IgG in SPR binding assays and values are listed for three IgG molecules.
| IgG | Uncomplexed IgG mean steady-state KD to FcRn (nM) | Antigen-complexed IgG mean steady-state KD to FcRn (nM) | Antigen-complexed/Uncomplexed fold change |
|---|---|---|---|
| mAb-2 | 10.7 ± 1.2 | 1024.6 ± 64.2 | 96 |
| mAb-3 | 68.6 ± 7.8 | 1062.5 ± 21.9 | 15 |
| mAb-4 | 379.4 ± 2.5 | 1060.0 ± 24.0 | 3 |