Table 2.
Description of panels of IgG molecules tested to identify domains of IgG that affect affinity to FcRn. Steady-state KD affinity values to FcRn were determined using the SPR binding assay for panels of IgG molecules with distinct characteristics.
| Panel | HC isotype | LC isotype | VH FW | VL FW | CDRs | Domain investigated | mAb |
|---|---|---|---|---|---|---|---|
| 1 | IgG1, 2 or 4 | Identical | Identical | Identical | Identical | HC isotype | 1, 8, 9 |
| 2 | Identical | Kappa or Lambda | Different | Different | Different | LC isotype | 2, 6, 10–13 |
| 3 | Identical | Identical | Different | Different | Different | FW family | 14–21 |
| 4 | Identical | Identical | Identical | Identical | Different: different targets | CDRs | 9–11, 22–24 |
| 5 | Identical | Identical | Identical | Identical | Different: same target and only 1–5 mutation differences | CDRs | 25–53 |
| 6 | Identical | Identical | Identical | Identical | Different: same target and only 1–3 mutation differences | CDRs | 54–58 |