Table 1.
Affinities and binding kinetics of MEDI7814. C5a affinity was estimated using surface plasmon resonance (BIAcore). C5 affinity was estimated by kinetic exclusion assay (KinExa). Affinity results are an average of 2 or more separate experiments. N.D. Not determined.
| Affinity Determination Method | Analyte | ka (M−1s−1) | kd (s−1) | Affinity KD (pM) |
|---|---|---|---|---|
| Surface plasmon resonance (BIAcore) | Recombinant human C5a | 1.74 × 107 | 2.51 × 10−4 | 14 |
| Serum purified human C5a | 2.18 × 107 | 1.65 × 10−4 | 8 | |
| Serum purified human C5adesR | 3.32 × 107 | 1.91 × 10−4 | 6 | |
| Serum purified Cynomolgus C5a | 8.08 × 106 | 1.54 × 10−4 | 19 | |
| Recombinant Cynomolgus C5adesR | 1.51 × 107 | 1.44 × 10−4 | 10 | |
| Kinetic exclusion assay (KinExa) | Recombinant human C5a | N.D. | N.D. | 4 |
| Serum purified human C5 | N.D. | N.D. | 27 |