HPLC analysis of N. fumigata easM knockout strain and representative transformant expressing C. gigantea cloA. Ergot alkaloids were detected by fluorescence with excitation and emission wavelengths of 272 and 372 nm, respectively. Abbreviations: easM ko, easM knockout; Trp, tryptophan; C.g., Claviceps gigantea; 3, festuclavine; 5, dihydrolysergic acid.