Figure 4.
Analysis of C. gigantea cloA mRNA expressed in N. fumigata easM knockout. (A) Qualitative reverse transcriptase-PCR analysis of mRNA from genomic clone of C. gigantea cloA transformed into N. fumigata easM knockout (lane 1) or fully processed clone of C. gigantea cloA expressed in N. fumigata easM knockout (lane 2). Sizes of relevant fragments of BstEII-digested bacteriophage λ DNA are indicated to the left of the image. (B) Representations of structures of C. gigantea cloA genomic DNA and the two versions of cloA mRNA that accumulated upon expression of the genomic C. gigantea cloA in N. fumigata easM knockout.
