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. 2017 Aug 17;39(2):230–242. doi: 10.1038/aps.2017.63

Figure 6.

Figure 6

Signaling involved in inhibiting osteoclast formation induced by QOA-8a. (A) Osteoclast precursors (RAW264.7 cells) were pretreated with vehicle (DMSO) or with 5 μmol/L QOA-8a and stimulated with RANKL in a time-course experiment, and Western blot analysis of ERK1/2, JNK, p38, p65, IκBα activating phosphorylation. (B) RAW264.7 cell cultures incubated on vehicle or with QOA-8a in the presence of RANKL for 1 or 2 d. Western blot analysis of c-Fos and NFATc1 proteins, normalized versus actin. (C) Real-time qPCR of c-Fos and NFATc1 expression, normalized with actin expression. Mean±SD. n=3. **P<0.01.