Table 1. Variation in the amounts of Bx (DIBOA and DIMBOA) in T. dicoccoides seedlings (nmol/mg FW) grown in Sapir greenhouse from seeds collected on the opposing slopes, AS and ES, of “Evolution Canyon”.
The measurements were separately done in shoots and roots of each seedling. Each average, standard error and median appearing in the table were calculated from 7 replications of the measuring at the same time. The replications appear in a separate column in the table. When the first 4 replications had 0 Bx, we terminated this analysis and assumed that the 3 unmeasured replications are also zero. Mean +/- Standard error values of the 7 replications measured each time, and Median values of the replications. Abbreviations: AS = African slope, ES = European slope, Bx = Benzoxazinoid. Glc = Glucosidase, Fw = Fresh Weight, nmol. = Nano mole, Rep. (or R.) = Replicates. Note: Each replication in the table is the result of measuring a mixture of 3 seedlings from the same genotype in order to receive more reliable amounts.
| AS seedlings | ES seedlings | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Hours after seeding | Shoot (nmol/mg FW) | Root (nmol/mg FW) | Shoot (nmol/mg FW) | Root (nmol/mg FW) | ||||||||||||
| Rep. | DIMBOA-Glc Mean ± SE (Median) |
Rep. | DIBOA-Glc Mean ± SE (Median) |
Rep. | DIMBOA-Glc Mean ± SE (Median) |
Rep. | DIBOA-Glc Mean ± SE (Median) |
Rep. | DIMBOA-Glc Mean ± SE (Median) |
Rep. | DIBOA-Glc Mean ± SE (Median) |
Rep. | DIMBOA-Glc Mean ± SE (Median) |
Rep. | DIBOA-Glc Mean ± SE (Median) |
|
| 24 | 0 0 0 0 |
0±0 (0) |
0.1 0.10.2 0 0.2 0 |
0.1±0.03 (0.1) |
0 0 0 0 |
0±0 (0) |
0.33 0.28 0.37 0.3 0.25 0.22 0.35 |
0.1±0.03 (0.1) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
| 30 | 0 0 0 0 |
0±0 (0) |
0.32 0.31 0.35 0.32 0.33 0.37 0.45 |
0.35±0.018 (0.33) |
0 0 0 0 |
0±0 (0) |
0.42 0.48 0.6 0.52 0.5 0.55 0.71 |
0.54±0.03 (0.52) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
0 0 0 0 |
0±0 (0) |
| 36 | 0 0 0 0 |
0±0 (0) |
0.5 0.7 1 0.4 0.5 0.3 0.3 |
0.528±0.09 (0.5) |
0 0 0 0 |
0±0 (0) |
1.2 0.8 0.8 0.7 1 1.2 1.2 |
0.98±0.08 (1) |
0.25 0 0.3 0.9 0 0.9 0.5 |
0.4±0.148 (1) |
0 0 0 0 |
0±0 (0) |
0.2 0.3 0.2 0.4 0 0.5 0.3 |
0.27±0.06 (0.3) |
1.5 1.2 1 0.2 0.4 0.6 0.9 |
0.82±0.12 (0.9) |
| 48 | 0 0 0 0 |
0±0 (0) |
2.7 3 2.5 2.4 2.1 3 1.8 |
2.5±0.16 (2.5) |
0 0 0 0 |
0±0 (0) |
6.5 7 5.4 6 6.1 5.9 6.5 |
6.2±0.195 (6.1) |
3 4 3.5 2.6 3 3 2.6 |
3±0.183 (3) |
0 0 0 0 |
0±0 (0) |
3 2 2 4 1.5 3 2 |
2.5±0.32 (2) |
6 6 3 4 5 5 7.4 |
5.2±0.43 (5) |
| 60 | 0 0 0 0 |
0±0 (0) |
1.7 2.1 1.9 1.1 1.5 1.4 0.8 |
1.5±0.17 (1.5) |
0 0 0 0 |
0±0 (0) |
6.6 7 7 75 7.3 5.54 7 |
6.5±0.329 (7) |
10 9 9 9 7 8 11 |
9±0.16 (9) |
1.7 2 1 1 1.5 1.3 2 |
1.5±0.16 (1.5) |
3 3 3 4 4 4 7 |
4±0.32 (4) |
10 10 7 8 9 8 11 |
9±0.56 (0) |
| 72 | 0 0 0 0 |
0±0 (0) |
0.5 0.4 0.3 0.5 0.4 0.5 0.9 |
0.5±0.07 (0.5) |
0 0 0 0 |
0±0 (0) |
5 4 6 4 4.3 5 3.9 |
4.6±0.291 (4.3) |
13 13 12 11 15 10 17 |
13±0.89 (13) |
2.5 2 3 2.5 2 3.5 3.4 |
2.7±0.232 (2.5) |
5 4 4 3 5 4 3 |
4±0.406 (4) |
12 15 13 13 9 14 15 |
13±0.507 (13) |
| 84 | 0 0 0 0 |
0±0 (0) |
0.4 0.4 0.8 0.6 0.5 0.5 |
0.6±0.804 (0.5) |
0 0 0 0 |
0±0 (0) |
3 3 5 4 3 4 6 |
4±0.435 (4) |
12.5 12 11 11 13 12 12.5 |
12±0.386 (15) |
3 3 3 4 5 4 6 |
4±0.435 (4) |
5 5 3 6 6 2.5 8.2 |
5.1±0.786 (5) |
10 12 14 12 11 14 9.6 |
11.8±0.665 (12) |
| 96 | 0 0 0 0 |
0±0 (0) |
0.5 0.4 0.3 0.4 0.5 0.4 0.3 |
0.4±0.03 (0) |
0 0 0 0 |
0±0 (0) |
5 5 4 3 2.5 5 4.9 |
4.2±0.401 (4.9) |
8 7 11 9 9 9 10 |
9±0.187 (9) |
12 10 7 9 10 10 12 |
10±0.654 (10) |
4 4 4 3 6 6 8 |
5±0.6 (4) |
8 8 6 5 7 7 8 |
7±0.436 (7) |
| 108 | 0 0 0 0 |
0±0 (0) |
0.3 0.3 0.3 0.2 0.2 0.4 0.4 |
0.3±0.03 (0.3) |
0 0 0 0 |
0±0 (0) |
2 3 4 3 3 3 3 |
3±0.218 (3) |
7 6 6 6 5 7 5 |
6±0.383 (6) |
9 9 9 7 10 8 11 |
9±0.487 (9) |
6 6.7 6 7 5.1 2 4.3 |
5.2±0.65 (6) |
7 7 8 6 6 5 6.5 |
6.5±0.37 (6.5) |
| 120 | 0 0 0 0 |
0±0 (0) |
0.4 0.4 0.5 0.2 0.2 0.3 0.8 |
0.4±0.07 (0.4) |
0 0 0 0 |
0±0 (0) |
3 2 2 4 2 3 2.9 |
2.7±0.09 (2.9) |
3 2 2 2 3 1.5 3.65 |
2.45±0.29 (2) |
8 8 8 6 5 9 12 |
8±0.845 (8) |
6 5.5 7 4 4 4 6.6 |
5.3±0.492 (5.5) |
5 5 4 3 7 2.5 3.6 |
3.4±0.06 (4) |
| 132 | 0 0 0 0 |
0±0 (0) |
0.4 0.5 0.1 0.3 0.2 0.2 0.4 |
0.3±0.03 (0.3) |
0 0 0 0 |
0±0 (0) |
3 3 2 2 2 3 2.5 |
2.5±0.108 (2.5) |
0.5 0.5 0.5 0.4 0.5 0.4 0.7 |
0.5±0.01 (0.1) |
6 5 9 7 7 7 8 |
7±0.74 (7) |
6 6 5 4 4 6 8 |
5.7±0.62 (6) |
4 2 2 2 2.5 2 3 |
2.5±0.32 (2) |
| 144 | 0 0 0 0 |
0±0 (0) |
0.4 0.4 0.4 0.2 0.2 0.3 0.3 |
0.3±0.03 (0) |
0 0 0 0 |
0±0 (0) |
3 3 3 2 2 2 1.8 |
2.4±0.213 (2) |
0.2 0 0 0.2 0.1 0.1 0.1 |
0.1±0.08 (0.1) |
5 5 5 4 7 6 3 |
5±0.48 (5) |
5 5 4 4 7 5 6.4 |
5.2±0.404 (5) |
3 3 2 1.5 1 2 2.2 |
2.1±0.245 (2) |