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. 2018 Feb 6;14(2):e1007178. doi: 10.1371/journal.pgen.1007178

Fig 1. Functional analysis of Axin domains required for its stabilization following Wingless stimulation.

Fig 1

Confocal images of stage 9 embryos expressing indicated transgene with the mat-Gal4 driver. Genotypes at left margin, antibodies on top. (A-C) By 40 minutes after the onset of Wg expression in segmental stripes, Axin-V5 is distributed in wide segmental stripes (brackets) that overlap with the Wg stripes (asterisks), indicating that Axin levels increase rapidly in cells responding to Wg exposure. (D-R) AxinΔTBD-V5 (D-F), AxinΔRGS-V5 (G-I), AxinΔPP2-V5 (M-O) and AxinΔDIX-V5 (P-R) are uniformly increased throughout the embryonic ectoderm; in contrast, AxinΔArm-V5 (J-L) is distributed in wide segmental stripes (brackets) that overlap the Wg stripes (asterisks). The Tankyrase-binding domain (TBD), Apc-binding domain (RGS), PP2A-binding domain (PP2), and Dishevelled-binding domain (DIX) are required for the initial accumulation of Axin in Wg-responding cells, whereas the Arm-binding domain (ARM) is dispensable for this process. Due to variation in staining intensity between embryos, the relative level of Axin in the stripes and interstripes can be assessed within a single embryo, but not between different embryos. For all images, anterior left, dorsal up.