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. 2018 Jan 25;14(1):e1006812. doi: 10.1371/journal.ppat.1006812

Fig 5. Effects of SP mutations on REJO virus capture and transmission by DC-SIGN.

Fig 5

(A) Parental Raji or Raji–DC-SIGN+ cells were incubated for 2 hours with WT or mutant viruses produced in 293T cells. Cells were washed extensively, and the amounts of p24 protein associated with the cells were measured by ELISA. (B) Parental Raji or Raji–DC-SIGN+ cells were incubated with WT and mutant viruses for 2 hours, washed to remove unbound viruses, and added to CD4+ TZM.bl cells. Viral transmission to the TZM-bl cells was determined by luciferase activity and calculated based on infection in TZM.bl cells without Raji cells as control (set to 100%). Background luciferase activity was determined in co-cultures without any virus. *, p< 0.05 as compared to WT (ANOVA). (C) Correlation of virus capture (top) and transmission (bottom) via DC-SIGN with the Env incorporation into the WT and mutant virions by Spearman’s rank test.