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. 2018 Jan 25;14(1):e1006812. doi: 10.1371/journal.ppat.1006812

Fig 11. Analysis of JRFL Env sugar moieties by lectin-probed Western blotting.

Fig 11

The same amounts of Env from JRFL WT and mutant viruses produced in 293T cells were separated by SDS-PAGE (10%) under reducing condition, blotted, and probed with an anti-gp120 MAb cocktail or lectins (GNA, GRFT, and AAL). A) Env reactivity with MAbs versus lectins. Two Env species with distinct molecular masses are indicated by red and green arrows. B) Density measurements of the upper and lower Env bands as recognized by anti-gp120 MAbs and different lectins. Density analysis was done by Image Lab software.