Fig 1. Selective depletion of splenic macrophages by i.v. delivery of clodronate liposomes (CLL).

Mice were left untreated (naïve) or injected i.v. with CLL or saline filled liposomes (PBSL) as a negative control; 3 days later spleens were harvested and cells analyzed by multi-color flow cytometry. (A) Representative plots showing CD11b vs. F4/80 staining of splenocytes from naïve and liposome treated mice. (B, C) Numbers for splenic and lymph node populations were calculated based as cell frequency X total spleen cell numbers. Data shown represent one of four separate experiments with similar results. Statistics were performed using 2-way ANOVA and Tukey’s multiple comparison test. P values: * p < 0.05, ** p <0.01, *** p<0.001, **** < 0.0001.