Fig 3. WNV-specific Ab responses in splenic MΦ-depleted mice.

Mice were treated as in Fig 2A with CLL (open) or PBSL (black), and sera were collected at days 5 and 8 p.i. (A) Anti-WNV Env IgM and IgG titers were determined by OD values that were 3 standard deviations above the mock controls. PNRT₅₀ represents the reciprocal dilution at which 50% of plaques were neutralized, The combination of two independent experiments is shown. (B) Serum samples were obtained from naïve, WNV immune (>15 days post-WNV), and at day 8 post-WNV from PBSL or CLL treated mice. Heat inactivated pooled sera were then injected into μMT B cell-deficient mice 1 day prior to and 1 day after inoculation of mice with 100 PFU WNV. A pool of sera from naïve mice was used a negative control and provided no protection. Two-tailed Student's t test, * p < 0.05, ** p <0.01, *** p<0.001.