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. 2018 Feb 6;13(2):e0192286. doi: 10.1371/journal.pone.0192286

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© 2018 Nemtsova et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — CHO cells were stably transfected with an AhdI fragment of pROSA26-Tg^LSL-TPP1 that contained only the CAG-LSL-TPP1 sequence. After selection with neo, CHO controls or cells containing CAG-LSL-TPP1 were transiently transfected with pCAG-cre and TPP1 activity measured at t = 0, 1 and 3 days. TPP1 activities were normalized to protein concentration and expressed as percentage of average CHO cell activity in the absence of CAG-LSL-TPP1. TPP1 activities in cells transfected with the transgene at 3 days were significantly elevated compared to all other conditions (p values ranging from 0.0003–0.0006 using Tukey’s multiple comparison test). All other comparisons had p values >0.7642.