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. 2018 Feb 6;13(2):e0192433. doi: 10.1371/journal.pone.0192433

Fig 1. Comparison of relative mCherry fluorescence in the supernatant of the studied expression vector constructs.

Fig 1

mCherry fluorescence was measured in the whole culture sample, compared with mCherry fluorescence in the supernatant, and is expressed as a percentage. mCherry fluorescence was measured from 96 individual colonies grown in a deep-well plate in TAP media for 7 days under constant illumination and agitation. Data presented in the boxplot were collected from colonies where the total fluorescence signal was higher than that of the auto fluorescence of the parental wild-type cc1690, within three standard deviations. pAH04 –construct without SP; pJP22 –construct with arylsulfatase 1 SP; pJP26 –construct with binding protein 1 SP; pJP28 –construct with carbonic anhydrase 1 SP; pJP29 –construct with ice-binding protein 1 SP; pJP30-35 –construct with in silico identified SP (iSP). n–number of positive signals obtained for each construct. * represents the average result. ○ represents the outliers.