Fig. 4.

16 rats underwent a memory reactivation session after 21 days of training on the T-maze, before brains were harvested for assessment of Zif268 expression. (A) (i): The rate of acquisition followed that of Experiment 1, although training was extended to 21 days to compare to the cannulated animals in Experiment 2. (ii): Latency to collect the reward decreased with training with no effect of strategy used at Test 1. (ii) Extended training of uncannulated rats resulted in a significant shift in the proportion of animals using a place strategy, ^***p < 0.001. (B) In samples taken from the pDLS, a non-significant increase in the expression of Zif268 relative to the 6 hours control group was seen in the rats using a response strategy, although the number of animals here is too small to draw a strong conclusion for the response group. The use of a place strategy did not appear to increase Zif268 expression in the hippocampus. The levels of Zif268 were not altered in the hippocampus by use of either strategy. Interestingly, there was an apparent increase in Zif268 levels in the BLA regardless of the strategy used at test. There was no significant difference in Zif268 levels after reactivation in the NAc, aDLS, aDMS nor pDMS. Bars are mean per group (Place 6H n = 5, Place 2H n = 6, Habit 2H n = 2) +/− SEM; images are representative blots.