Fig. 10.6.

Architecture of the CMGE complex. (a) The Pol2 (P2) subunit of Pol ε is comprised of a catalytic N-terminal subdomain and an inactive B-family Pol in the C-terminal subdomain. (b) Negative stain 3D EM map of the CMGE in four different views (EMD-6465). The EM density is segmented and colored according to individual subunits except for the Pol ε where the complex is shown in green. Part of the Pol ε density is not visible in the EM and may be the N-terminal subdomain of Pol2 as proposed in Sun et al. (2015). (c) Cross-linking mass spectrometry identified multiple contacts between Pol2 and GINS, Cdc45, and the CTD regions of Mcm2, Mcm5, and Mcm6 (This figure is modified with permission from Figure 6 of Yuan et al. 2016)