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. 2018 Feb 5;217(2):527–540. doi: 10.1083/jcb.201704028

Figure 4.

Figure 4.

MMSET is required for efficient NER. (A) MMSET is required for efficient NER. The graph shows relative EdU incorporation as a result of UDS in MRC5 cells transfected with the indicated esiRNAs, XPA-deficient fibroblasts, and CSA-deficient fibroblasts (mean ± SEM). EdU intensity was measured in three independent experiments, with 200 cells counted per experiment. Statistical significance was assessed by an unpaired t test. **, P ≤ 0.001; ***, P ≤ 0.0001. (B) MMSET is required for efficient NER. RRS assay showing levels of EU incorporation in the indicated cells in unexposed cells and cells exposed to UV damage. The EU intensity is normalized to the intensity of the −UV condition for each cell type. EU intensity was measured for 200–300 cells per experiment (n = 3). (C) Knockdown of MMSET impairs cell survival of UV-exposed cells. The graph shows the relative cell viability (as measured by MTT assay at 72 h after UV) of U2OS cells with stable shRNA knockdowns of XPC and MMSET and control cells (shNMC) at the indicated UV dose (n = 3).