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. 2018 Feb 5;217(2):763–777. doi: 10.1083/jcb.201705031

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© 2018 Viotti et al.

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Figure 1. — Genetic screen identifies SUV420H2 as a modulator of epithelial/mesenchymal states in pancreatic cancer. (A) Diagram of arrayed siRNA screen to identify epigenetic factors modulating epithelial/mesenchymal states in pancreatic cancer. (B) Screen results for each marker depicting hit selection criteria. Only factors for which at least two of four siRNAs resulted in values in the region of interest (represented by dashed boxes) were considered for hit selection. Dotted vertical lines indicate demarcation of statistical significance (P < 0.01). Averaged results of nontargeting siRNA control (NTC) knockdown (negative control) are set as the reference (Z score = 0), olive-colored bars and asterisks indicate averaged results for ZEB1 knockdown (positive control), and pink bars and asterisks indicate values for SUV420H2 knockdown. (C) Summary of epigenetic factors that on knockdown elicit a highly significant change in marker signal, as established by selection criteria in B. (D) Original screen images for NTC and SUV420H2 knockdown depicting de novo, cell-junction localizing the E-CAD and EPCAM immunofluorescence signal, as well as the reduced VIM signal for the latter. Bars, 50 µm.