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. 2018 Feb 5;217(2):701–714. doi: 10.1083/jcb.201705160

Figure 6.

Figure 6.

scar/wasp mutant cells retain the ability to make blebs. (A) Cells of indicated genotype expressing LifeAct-mRFP (red) and GFP-ArpC4 (green) were imaged by spinning disc microscopy (Andor Revolution XD) during chemotaxis while highly compressed. White arrows highlight blebs. Bar, 10 µm. (B) Highly compressed scar/wasp mutant cell expressing LifeAct-mRFP (red) and GFP-ArpC4 (green), imaged using spinning disc microscopy (Andor Revolution XD). White arrows indicate blebs. Bar, 10 µm. (C and D) Laser ablation of actin cortex induced blebbing in uncompressed cells. (C) Indicated cells expressing LifeAct-mRFP shown before, 0 s, 10 s and 90 s after laser ablation. White dot indicates site of ablation. White box in preablation image enlarged in subsequent post-ablation images. Bars, 10 µm (left showing whole cells); 4 µm (insets). (D) Percentage of cells for each genotype that blebbed in response to laser ablation of the cortex (n = 30). Only scar/wasp double mutants significantly differed from the controls (chi-square test, P < 0.0001) but still induced blebbing ∼20% of the time.