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. 2018 Feb 6;7:e32637. doi: 10.7554/eLife.32637

Figure 6. Chemical ablation of mechanosensory lateral line reduces HCRT levels in Pachón cavefish.

Figure 6.

(A-D). Photomicrographs of surface fish cranial regions stained with DASPEI to reveal lateral line mechanosensory neuromasts. Treatment with gentamicin ablates lateral line neuromasts in surface fish (B) and Pachón cavefish (D) (E) Gentamicin treatment has no significant effect on hcrt expression in surface fish (p>0.635, n = 8) while in Pachón cavefish gentamicin treatment significantly reduces hcrt expression, restoring surface-like levels. (Pachón treated vs. untreated, p<0.0001; Pachón treated vs. surface untreated, p>0.635, n = 8, F(1,28)=21.28). (F) Fluorescent intensity per hypothalamic HCRT-cell was not altered with gentamicin treatment in surface fish, p=0.590, n = 4. In Pachón cavefish, HCRT neuropeptide levels are significantly lower following gentamicin treatment (p<0.0001, n = 4; 2-way ANOVA, F (1, 13)=0.0001 G. Gentamicin treatment has no effect on total number of HCRT cell number in either surface or Pachón cavefish (p>0.494, n = 8, F (1, 13C)=0.4967). (H–K) Representative 2 μm confocal images of the dorsal hypothalamic region in surface fish and Pachón cavefish immunostained with HCRT (green) and DAPI (white) (H) Surface control (I) Pachón control (J) Surface gentamicin K. Pachón gentamicin. Scale bar = 50 μm.

Figure 6—source data 1. Chemical ablation of mechanosensory lateral line reduces HCRT levels in Pachón cavefish.
This source file contains analysis of the effects of lateral line ablation with gentamicin on hcrt expression (Figure 6E), HCRT cell number (Figure 6F), and HCRT fluorescence levels (Figure 6G).
elife-32637-fig6-data1.xlsx (10.6KB, xlsx)
DOI: 10.7554/eLife.32637.025