Figure 5.

Overexpression of αv integrin in H520 NSCLC in vitro increases tumor occurrence after intracerebral inoculation. A) αv integrin protein level in H520 cells including parental, vector only or αv integrin knock-in (avINT_ki) clones with tubulin as loading control. B) Brain immunohistochemistry of reprensentative rats stainned with human mitochondrial antigen; Athymic rats were intracerebrally inoculated with 10⁶ H520 avINT-RFP#2 and –RFP alone, brains were harvested at 56 days after inoculation. C) Dot plot chart of individual brain tumor volume and number of rats with tumor after inoculation shown shown in Figure 4B.