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(A-B) Mosaic-labeled KV in a control embryo (A) and an embryo treated with ouabain from 4ss to 6ss (B). (C) Measurements of maximum KV lumen area indicated that ouabain treatments between 4-6 ss were not effective at blocking KV lumen expansion. N=number of embryos analyzed. Error bars=one standard deviation. ns=not significant, (Welch’s T-Test).
