Figure 1.

Effect of histone deacetylase (HDAC) inhibitors on expression of extracellular superoxide dismutase (EC-SOD) and NADPH oxidase (NOX) 4 genes in pulmonary artery cells. Cells were incubated with the indicated concentrations of HDAC inhibitors for 24 hours. Total RNA was isolated from human pulmonary artery endothelial cells (HPAECs) (A and B) or human pulmonary artery smooth muscle cells (C and D), and gene-specific messenger RNA (mRNA) levels were analyzed using quantitative RT-PCR. EC-SOD and NOX4 mRNA levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. The results are shown as mean ± SD (for primer sequences, see Table 1). *P < 0.001 and ^#P < 0.05 when compared with cells treated with DMSO (one-way ANOVA and Bonferroni test).