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. 2017 Dec 27;9(7):7359–7371. doi: 10.18632/oncotarget.23670

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Copyright: © 2018 Binato et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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To confirm the obtained chip array results, RT-qPCR was used to analyze selected differentially expressed genes using a larger number of Brazilian patient samples to determine changes in mRNA expression levels after normalization to Actin and GAPDH. RT-qPCR analyses of MMP7 (A), SPARC (B) and TIMP1 (C) (overexpressed in patients with intestinal gastric cancer) and PGA4 (D), KRT20 (E), AKR1C2 (F), GIF (G) and CHGA (H) (downregulated in patients with intestinal gastric cancer) confirmed the chip array assay results and the common molecular signature that was able to discriminate all tumor tissues from all intestinal-type gastric carcinoma patients from non-tumor control tissue. ^*p < 0.05; ^**p < 0.01.