Table 1. mRNA expressions of FOXO in breast cell lines and tumors.
| Cell line/ Tumor | Gene | FOXO1 | FOXO3 | FOXO4 | FOXO6 |
|---|---|---|---|---|---|
| Expression | Number (%) | ||||
| Tumorigenic cell lines (n = 32) | Overexpression Normal expression |
0 (0) 32 (100) |
0 (0) 32 (100) |
0 (0) 32 (100) |
8 (25) 24 (75) |
| Tumors (n = 527) |
Overexpression Normal expression |
1 (0.2) 526 (99.8) |
23 (4.4) 504 (95.6) |
6 (1.1) 521 (98.9) |
142 (26.9) 385 (73.1) |
FOXO6-expression values of the breast cell lines were normalized so that the ‘basal FOXO6 mRNA level’ (smallest quantifiable amount of mRNA (Ct = 35)) was equal to 1. Overexpression was defined as Ct values under 30 (values above 32 (2ΔCt = 235–30 = 32)).
FOXO6-expression values of the breast tumors were normalized so that the median FOXO6-expression value of normal breast tissues was equal to 1. Overexpression was defined as threefold variations of expression relative to the median expression of normal samples.