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. 2018 Jan 8;9(7):7541–7556. doi: 10.18632/oncotarget.24045

Figure 1. ANT2 is a potential therapeutic target for DIPG.

Figure 1

(A) Representative western blot image of ANT2 protein in a panel of DIPG cells and normal astrocytes (NHA); (B) Cytotoxic efficacy of PENAO tested for 72 h against a panel of neurosphere-forming DIPG cells. Viability was assessed by resasurin assay and presented as a percentage viability compared to untreated cells; Experiment was replicated 3 times; (C) Flow cytometric analysis of HSJD-DIPG007 cells for production of mitochondrial ROS. DIPG cells were treated with increasing concentration of PENAO (P) for 18 h and subsequently stained with MitosoxRed and analysed with FACS Canto B. Data represent average and SD of 3 determinations; untreated vs PENAO p < 0.001 for all concentrations tested; For positive control DIPG cells were treated with 100 uM antimycin for 1 h and analysed. (D) Flow cytometric analysis of HSJD-DIPG007 cells for mitochondrial depolarisation. DIPG cells were treated with increasing concentration of PENAO for 18 h and subsequently stained with JC1 and analysed with FACS Canto B. Data represent average and SD of 3 determinations; untreated vs PENAO p < 0.01 for all concentrations tested. For positive control DIPG cells were treated with 50 uM CCCP for 2 h and analysed.