Table 3.
Associations between urinary concentration of DINCH metabolites, metabolites of high molecular weight phthalates, and ΣDEHP and intermediate outcomes of assisted reproduction (n=136 women/cycles).
| Adjusted Mean (95% CI)1
|
|||||
|---|---|---|---|---|---|
| SG-adjusted Biomarker Concentration (Range) | N | Total Oocytes | Mature Oocytes | Fertilized Oocytes | Top Quality Embryos |
| Σ DEHP (μmol/L) | |||||
| T1 (0.02–0.12) | 45 | 11.4 (10.4, 12.4) | 8.8 (8.0, 9.7) | 5.9 (5.3, 6.7) | 3.0 (2.5, 3.5) |
| T2 (0.12–0.22) | 46 | 9.0 (8.1, 9.9)* | 7.3 (6.6, 8.1)* | 5.0 (4.4, 5.7) | 2.2 (1.8, 2.7)* |
| T3 (0.22–2.65) | 45 | 8.5 (7.7, 9.4)* | 7.1 (6.4, 7.9)* | 4.7 (4.1, 5.4)* | 1.9 (1.5, 2.3)* |
| P-trend2 | <0.001 | 0.02 | 0.02 | 0.002 | |
| MEHP (μg/L) | |||||
| T1 (0.00–2.71) | 45 | 9.8 (8.9, 10.7) | 7.3 (6.6, 8.2) | 4.8 (4.2, 5.5) | 2.5 (2.0, 3.0) |
| T2 (2.72–6.07) | 46 | 10.1 (9.3, 11.1) | 8.1 (7.3, 9.0) | 5.6 (5.0, 6.4) | 2.5 (2.1, 3.0) |
| T3 (6.08–70.6) | 45 | 8.9 (8.0, 9.8)† | 7.8 (7.0, 8.6) | 5.2 (4.6, 5.9) | 2.1 (1.7, 2.6) |
| P-trend | 0.07 | 0.69 | 0.78 | 0.19 | |
| MEHHP (μg/L) | |||||
| T1 (2.10–9.73) | 45 | 11.6 (10.7, 12.7) | 9.0 (8.1, 9.9) | 6.0 (5.3, 6.8) | 2.9 (2.4, 3.4) |
| T2 (9.74–17.9) | 46 | 9.3 (8.5, 10.3)* | 7.5 (6.8, 8.3)* | 5.1 (4.5, 5.8) | 2.3 (1.9, 2.8) |
| T3 (18.0–215) | 45 | 7.9 (7.1, 8.7)*† | 6.7 (6.0, 7.5)* | 4.6 (4.0, 5.2)* | 1.9 (1.5, 2.4)* |
| P-trend | <0.001 | <0.001 | 0.006 | 0.006 | |
| MEOHP (μg/L) | |||||
| T1 (1.40–7.49) | 45 | 11.6 (10.6, 12.7) | 9.0 (8.1, 9.9) | 6.1 (5.4, 6.9) | 3.0 (2.5, 3.6) |
| T2 (7.50–13.3) | 46 | 9.2 (8.4, 10.2)* | 7.5 (6.8, 8.3)* | 5.1 (4.5, 5.8)* | 2.2 (1.8, 2.7)* |
| T3 (13.4–145) | 45 | 8.0 (7.2, 8.8)*† | 6.7 (6.0, 7.5)* | 4.5 (3.9, 5.1)* | 1.9 (1.5, 2.3)* |
| P-trend | <0.001 | <0.001 | 0.002 | 0.002 | |
| MECPP (μg/L) | |||||
| T1 (1.68–15.3) | 45 | 11.0 (10.1, 12.0) | 8.3 (7.5, 9.2) | 5.5 (4.8, 6.2) | 2.5 (2.1, 3.1) |
| T2 (15.4–26.3) | 45 | 9.4 (8.5, 10.3)* | 7.8 (7.0, 8.6) | 5.5 (4.8, 6.2) | 2.8 (2.4, 3.3) |
| T3 (26.4–371) | 46 | 8.5 (7.7, 9.4)* | 7.1 (6.4, 7.9)* | 4.8 (4.2, 5.4) | 1.8 (1.4, 2.2) |
| P-trend | <0.001 | 0.03 | 0.10 | 0.004 | |
| MBzP (μg/L) | |||||
| T1 (0.00–1.23) | 45 | 9.6 (8.7, 10.6) | 7.6 (6.9, 8.5) | 5.2 (4.6, 5.9) | 2.5 (2.1, 3.0) |
| T2 (1.24–2.35) | 46 | 9.5 (8.7, 10.4) | 7.7 (7.0, 8.6) | 5.0 (4.4, 5.7) | 2.2 (1.8, 2.6) |
| T3 (2.35–382) | 45 | 9.7 (8.8, 10.6) | 7.8 (7.1, 8.7) | 5.4 (4.8, 6.2) | 2.5 (2.0, 3.0) |
| P-trend | 0.86 | 0.74 | 0.57 | 0.93 | |
| MCPP (μg/L) | |||||
| T1 (0–0.91) | 44 | 8.9 (8.1, 9.9) | 7.2 (6.4, 8.0) | 4.8 (4.2, 5.5) | 2.4 (1.9, 2.9) |
| T2 (0.92–1.68) | 46 | 10.1 (9.3,11.1) | 8.3 (7.5, 9.2) | 5.6 (5.0, 6.4) | 2.6 (2.2, 3.1) |
| T3 (1.69–131) | 46 | 9.7 (8.8, 10.6) | 7.7 (6.9, 8.6) | 5.2 (4.6, 5.9) | 2.2 (1.8, 2.6) |
| P-trend | 0.48 | 0.62 | 0.64 | 0.37 | |
| MCOP (μg/L) | |||||
| T1 (1.68–6.15) | 45 | 9.2 (8.3, 10.1) | 7.2 (6.4, 8.0) | 5.0 (4.4, 5.7) | 2.5 (2.0, 3.0) |
| T2 (6.16–11.14) | 46 | 10.2 (9.3, 11.2)* | 8.4 (7.6, 9.3)* | 5.7 (5.0, 6.4) | 2.5 (2.1, 3.0) |
| T3 (11.15–1344) | 45 | 9.4 (8.5, 10.3) | 7.6 (6.8, 8.4) | 5.0 (4.4, 5.7) | 2.1 (1.7, 2.6) |
| P-trend | 0.75 | 0.91 | 0.51 | 0.20 | |
| MiNP (μg/L) | |||||
| T1 (<LOD) | 66 | 9.9 (9.2, 10.7) | 7.8 (7.2, 8.5) | 5.2 (4.7, 5.8) | 2.6 (2.2, 3.0) |
| T2 (0.50–1.40) | 35 | 9.5 (8.5, 10.5) | 7.6 (6.8, 8.6) | 5.0 (4.4, 5.8) | 2.1 (1.7, 2.7) |
| T3 (1.41–263) | 35 | 9.2 (8.2, 10.2)* | 7.6 (6.7, 8.6) | 5.4 (4.7, 6.3) | 2.1 (1.7, 2.7) |
| P-trend | 0.28 | 0.74 | 0.63 | 0.18 | |
| MONP (μg/L) | |||||
| T1 (0.56–2.79) | 45 | 9.8 (8.9, 10.7) | 7.7 (6.9, 8.5) | 5.3 (4.6, 6.0) | 2.5 (2.0, 3.0) |
| T2 (2.80–4.83) | 46 | 9.4 (8.5, 10.3) | 7.6 (6.8, 8.4) | 4.9 (4.3, 5.6) | 2.4 (2.0, 2.9) |
| T3 (4.84–902) | 45 | 9.7 (8.8, 10.6) | 7.9 (7.2, 8.8) | 5.5 (4.9, 6.3) | 2.2 (1.8, 2.7) |
| P-trend | 0.98 | 0.57 | 0.41 | 0.48 | |
| MCNP (μg/L) | |||||
| T1 (0.21–0.83) | 47 | 10.1 (9.2, 11.1) | 7.8 (7.1, 8.7) | 5.3 (4.7, 6.0) | 2.4 (2.0, 2.9) |
| T2 (0.84–1.67) | 44 | 9.0 (8.2, 10.0) | 7.2 (6.4, 8.1) | 4.9 (4.2, 5.6) | 2.0 (1.6, 2.5) |
| T3 (1.68–46.0) | 45 | 9.6 (8.7, 10.6) | 8.1 (7.3, 9.0) | 5.5 (4.9, 6.2) | 2.7 (2.2, 3.2) |
| P-trend | 0.76 | 0.36 | 0.44 | 0.22 | |
| MHiNCH (μg/L) | |||||
| T1 (0.19–0.91) | 45 | 10.1 (9.2, 11.1) | 8.0 (7.2, 8.9) | 5.0 (4.4, 5.7) | 2.0 (1.6, 2.4) |
| T2 (0.92–1.77) | 45 | 8.3 (7.5, 9.2)* | 6.6 (5.9, 7.4)* | 4.7 (4.1, 5.3) | 2.5 (2.0, 3.0) |
| T3 (1.78–74.8) | 46 | 10.3 (9.4, 11.3) | 8.6 (7.8, 9.5) | 6.0 (5.3, 6.8)* † | 2.6 (2.2, 3.2)* |
| P-trend | 0.20 | 0.06 | 0.008 | 0.07 | |
| MCOCH (μg/L) | |||||
| T1 (0–0.45) | 45 | 9.0 (8.1, 9.9) | 7.4 (6.6, 8.2) | 4.7 (4.1, 5.4) | 2.3 (1.9, 2.8) |
| T2 (0.46–0.84) | 45 | 10.4 (9.5, 11.4) | 8.0 (7.2, 8.9) | 5.4 (4.7, 6.1) | 2.1 (1.7, 2.6) |
| T3 (0.85–26.6) | 46 | 9.5 (8.6, 10.4) | 7.8 (7.0, 8.6) | 5.6 (4.9, 6.3) | 2.6 (2.2, 3.2) |
| P-trend | 0.97 | 0.70 | 0.15 | 0.18 | |
| MEHHTP (μg/L) | |||||
| T1 (0–1.62) | 45 | 10.0 (9.1, 11.0) | 7.9 (7.1, 8.8) | 5.3 (4.6, 6.0) | 2.3 (1.9, 2.8) |
| T2 (1.63–3.71) | 46 | 9.6 (8.7, 10.5) | 7.8 (7.0, 8.7) | 5.3 (4.6, 6.0) | 2.6 (2.1, 3.1) |
| T3 (3.72–386) | 45 | 9.3 (8.4, 10.2) | 7.5 (6.7, 8.3) | 5.1 (4.5, 5.8) | 2.2 (1.8, 2.7) |
| P-trend | 0.32 | 0.47 | 0.75 | 0.54 | |
| MECPTP (μg/L) | |||||
| T1 (0.84–5.53) | 45 | 8.8 (8.0, 9.7) | 6.9 (6.1, 7.7) | 4.6 (4.0, 5.2) | 2.0 (1.6, 2.5) |
| T2 (5.54–13.25) | 45 | 10.0 (9.2, 11.0) | 8.3 (7.5, 9.2)* | 5.6 (5.0, 6.3)* | 2.6 (2.2, 3.1) |
| T3 (13.26–2856) | 46 | 9.9 (9.1, 10.9) | 8.0 (7.2, 8.8) | 5.5 (4.8, 6.2)* | 2.5 (2.0, 2.9) |
| P-trend | 0.19 | 0.21 | 0.16 | 0.40 | |
1
Models were run using Poisson regression with log link. All data are presented as adjusted mean counts controlling for maternal age, body mass index, and current smoking status.
*
p-value for T3 or T2 vs. T1 < 0.05;
†
p-value for T3 vs. T2 < 0.05