Table 2.
Intracellular delivery score (IDS) larger than 9 were defined as optimal treatment conditions using propidium iodide (PI) for the delivery efficiency (D) and cell membrane permeability (P) and LIVE/DEAD Cell Imaging kit for cell viability (V).
Intracellular delivery score (IDS) were defined as optimal treatment conditions for small-molecules, which enabled simultaneous satisfaction of high percentage of delivery efficiency and high cell membrane permeability with minimal influence to cells.
| HeLa | MCF-7 | MCF-10A | MDA-MB231 | ||||||||||||
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| Vpp (V) | Tt (μs) | IDS | Vpp (V) | Tt (μs) | IDS | Vpp (V) | Tt (μs) | IDS | Vpp (V) | Tt (μs) | IDS | ||||
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| 4 hr | 20 hr | 4 hr | 20 hr | 4 hr | 20 hr | 4 hr | 20 hr | ||||||||
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| 18 | 60 | 9 | 9 | 18 | 90 | 10 | 10 | 21 | 60 | 9 | 9 | 18 | 60 | 10 | 10 |
| 18 | 90 | 9 | 9 | 21 | 60 | 10 | 10 | 24 | 30 | 9 | 9 | 21 | 30 | 9 | 9 |
| 21 | 60 | 10 | 10 | 24 | 30 | 9 | 9 | 27 | 18 | 9 | 9 | 24 | 18 | 10 | 10 |
| 24 | 30 | 9 | 9 | 27 | 18 | 9 | 9 | 27 | 30 | 9 | 9 | 24 | 30 | 10 | 10 |
| 27 | 30 | 10 | 10 | 27 | 30 | 10 | 10 | - | - | - | - | 27 | 12 | 9 | 9 |
| - | - | - | - | - | - | - | - | - | - | - | - | 27 | 18 | 10 | 10 |