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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Biochemistry. 2018 Jan 4;57(5):620–630. doi: 10.1021/acs.biochem.7b01040

Figure 4. EAS of A79Q-EL222.

Figure 4

from global fitting of the data showing (A) 1FMN*, (B) 3FMN* and (C) A390 of EL222 and A79Q-EL222. Changes in ring modes at 1544 cm−1, 1380 cm−1, and 1425 cm−1 indicate that hydrogen bonding to FMN is restored compared to wild-type EL222.