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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Nov 21;1862(3):567–575. doi: 10.1016/j.bbagen.2017.11.017

Figure 4.

Figure 4

Production and characterization of human IGF-I prepropeptide 1A with a C-terminal flag tag. Bovine chondrocytes were transfected by pAAV-IGF-I1A-flag (1A-flag). The human IGF-I product was assessed in day 2 conditioned medium (CM) by western blotting of CM samples probed with anti-IGF-I antibody (A) or anti-flag antibody (B). Recombinant IGF-I (rIGF-I) and pAAV-IGF-I1A transfection were included for comparison. The data are from a representative experiment and equal amounts (40 ul) of CM were loaded for each sample. Human recombinant IGF-I (rIGF-I, 2 ng) was loaded for comparison. The data shows that the cells transfected with pAAV-IGF-I1A-flag produced mature IGF-I and two forms of IGF-I propeptide A (proIGF-IA) with flag tag. The cells transfected with pAAV-IGF-I1A produced mature IGF-I and two forms of proIGF-IA.