Fig. 3.
Anti-tumor activity of the BRAF peptide vaccine in murine BRAF-mutant model. Mice were subcutaneously inoculated with either 2 × 105 (a) or 1 × 106 (b) BPD6 cells on day 0. Vaccination with 5% glucose (The PBS group), empty LCP (The Empty group), LCP-CpG (The CpG group), LCP-BRAF peptide (The BRAF group), or LCP-(BRAF + CpG) (The BRAF + CpG group) was given on day 10. Tumor growth was measured every 2–3 days. Five mice from each group were killed on day 29, and tumors, whole blood, and organs were harvested for further study. n = 5, *p < 0.05, **p < 0. 01. Preventive vaccination (n = 10) significantly reduced tumor growth compared with PBS control (n = 8), with T/C ratio of 22.3%. Two animals in the vaccinated group did not grow tumors (c)