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. Author manuscript; available in PMC: 2019 Feb 5.
Published in final edited form as: Dev Cell. 2018 Jan 18;44(3):337–347.e5. doi: 10.1016/j.devcel.2017.12.022

Fig. 5. Edn1 inhibits Nr2f expression in the mandibular prominence.

Fig. 5

(A) edn1 is expressed comparably in the ventral ectoderm of wild-type controls and mutants at 20 hpf (arrowheads) and 30 hpf (magenta). (B) Expression of the Edn1 receptors is reduced (ednraa) or largely unaffected (ednrab) in Nr2f mutants. Arch NCCs are labeled with dlx2a (green), and nr2f1b genotypes are indicated. (C) In edn1 mutants, expression of all four Nr2f genes (magenta) ectopically increases to varying degrees in the mandibular domain (white arrows). Misexpression of edn1 induced by a heat shock of hsp70l:Gal4; UAS:Edn1 embryos from 20–24 hpf reduced Nr2f expression throughout the arches. Images are maximum intensity projections. Merged images with dlx2a arch NCC staining are presented in Fig. S5. Scale bars: A (top panel) = 100 μm; A (bottom panel), B, C = 20 μm.