Table 1.

Top 20 Nr2f-inhibited genes.

Gene	full name	nr2f2/5 mutant vs. control	nr2f5 OE vs. control	nr2f5 OE vs. nr2f2/5 mutant	Edn1 regulation
dlx4b	distal-less homeobox 4b	+1.15	n.d. in OE	n.d. in OE	activated
fgfbp2a	fibroblast growth factor binding protein 2a	+1.86	n.d. in OE	n.d. in OE	activatedd
six5	sine oculis-related homeobox 5	+1.51	−25.67	−38.69	-
dlx5a	distal-less homeobox 5a	+1.68	−8.57	−14.40	activateda
Nkx3.2	NK3 homeobox 2	+1.80	−6.75	−12.18	activatedb
dlx4a	distal-less homeobox 4a	+1.25	−9.32	−11.64	activated
Sim2	single-minded homolog 2	+1.25	−9.10	−11.34	-
TWSG1	twisted gastrulation BMP signaling modulator 1	+1.40	−8.01	−11.19	activated
f3b	coagulation factor IIIb	+1.75	−5.33	−9.32	activated
hand2	heart and neural crest derivatives expressed transcript 2	+1.91	−4.72	−9.04	activatedc
zgc:110626	protein-coding gene	+2.17	−3.84	−8.12	activated
barx1	BARX homeobox 1	+1.57	−5.03	−7.90	activateda
dlx3b	distal-less homeobox	+1.27	−5.48	−6.97	activatedc
Lrrc8c	leucine rich repeat containing 8 family, member C	+1.60	−3.76	−6.03	activated
sema3bl	semaphorin 3bl	+1.47	−3.93	−5.77	activatedd
Msx1a	muscle segment homeobox 1a	+1.80	−2.91	−5.24	activatedc
Nog2	noggin 2	+1.60	−3.02	−4.84	activated
cited2	Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2	+5.50	+1.16	−4.73	-
Hsd11b2	hydroxysteroid (11-beta) dehydrogenase 2	+2.36	−1.95	−4.60	-
Foxf2a	forkhead box F2a	+1.74	−2.62	−4.56	-

n.d., not detected. Unless otherwise referenced, Edn1 regulation was determined using published RNAseq data (Askary et al., 2017), with Edn1 activated and inhibited genes showing overexpression/control fold-change: mutant/control fold-change TPM ratios > 1.5 and < 0.667, respectively. A dash indicates no clear activation or repression. Predicted regulation confirmed by in situ:

^aWalker et al. 2006;

^bMiller et al. 2003;

^cMiller et al. 2000;

^dAskary et al. 2017. Also see Table S2 for Nr2f-activated genes.