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Kinetic constants for 26-hydroxylation of cholesterol and cholest-4-en-3-one by purified CYP124A1, CYP125A1, and CYP142A1. KD is the spectroscopically determined binding constant.
| Substrate | Enzyme | KD (nM) | Kmapp (μM) | kcatapp (min−1) | kcatapp/Kmapp (μM−1 min−1) |
|---|---|---|---|---|---|
| Cholesterol | 124A1 | - | 11.6 | 1.5 | 0.13 |
| 125A1 | 107 | 10.7 | 28 | 2.6 | |
| 142A1 | 18.4 | 7.7 | 16.7 | 2.2 | |
|
| |||||
| Cholest-4-en-3-one | 124A1 | 1056 | 20.8 | 11.7 | 0.56 |
| 125A1 | 1180 | 20.8 | 175 | 8.4 | |
| 142A1 | 114 | 11.8 | 84 | 7.1 | |
