Expression of proton-coupled oligopeptide transporters (POTs) in the colon and immune cells. (A) mRNA expression of POTs in proximal and distal colon, epithelial cells, lamina propia mononuclear cells (LPMCs), CD11b+ macrophages, and CD11b− immune cells prepared from mouse colon. The CT values of POTs genes and Gapdh ranged from 16~29. Data are expressed as mean ± SE (n=3). Protein expression of PEPT1 (B) and PEPT2 (C) in distal colon (DC), and epithelial cells (EC), lamina propia mononuclear cells (LPMCs), and CD11b+ macrophages prepared from mouse colon. The small intestine was used as a positive control for PEPT1, the kidney was used as a positive control for PEPT2. Immunoblots are shown for one representative experiment from three independent studies showing similar results.