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MDCK-hPHT219,20AA and mock cells were incubated in MES buffer (pH 5.5) with 25 μg/mL of MDP (A) or tri-DAP (C) for 30 or 60 min at 37°C. MDCK-hPHT219,20AA and mock cells were incubated in MES buffer (pH 5.5) with 10 μg/mL and 25 μg/mL of MDP (B) or tri-DAP (D) for 30 min at 37°C. Data are expressed as mean ± SE (n=3). **p < 0.01 and ***p < 0.001 as compared to mock cells using an unpaired Student’s t-test.
