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musPHT2 expression in RAW264.7 cells evaluated by qRT-PCR and western blot. RAW264.7 cells were transiently transfected with pcDNA3.1(+)-musPHT2-his tag or vector for 24 hr, total RNA and protein were isolated respectively for qRT-PCR (A) and western blot (B) to evaluate the expression of PHT2. Data are expressed as mean ± SE (n=3). Immunoblots are shown for one representative experiment from three independent studies showing similar results.
